FIGURE 5.

GlcNAc induces the catabolic genes in the mutant cells. A, expression of the GlcNAc transporter (NGT1), GlcNAc-6-PO₄ deacetylase (DAC1), and glucosamine-6-PO₄ deaminase (NAG1) genes. The graphs for the wild-type control, hxk1Δ, and h-d strains are indicated on the left. Cells were grown in medium containing 50 mm dextrose (d) or 50 mm N-acetylglucosamine (n) for 2 h at 37 °C. B, relative expression of NGT1, the GlcNAc-induced gene GIG1, and PMA1. The level of expression for each gene was normalized to that for the wild-type control strain grown in dextrose. Note that PMA1 is a control gene that is not regulated by GlcNAc. The mRNA levels were analyzed by qPCR as described under “Experimental Procedures.” The results represent the mean ± S.D. of four independent assays each carried out in triplicate. The data include analyses of two completely independent mutant isolates and RNA preparations for each strain.