FIGURE 6.

GlcNAc induction of Ngt1-GFP in hxk1Δ and h-d mutants. Analysis of Ngt1-GFP production in cells grown overnight in dextrose medium to early log phase (low cell density) and then switched to medium containing 50 mm dextrose (A) or 50 mm GlcNAc (B) for 2 h. C, cells grown overnight to saturation (High cell density) in medium containing 50 mm dextrose. The cells were then photographed using a fluorescence microscope to detect GFP. A gene fusion between the GlcNAc transporter and the green fluorescent protein (NGT1-GFP) was introduced into cells to create a wild-type control strain (SN787), an hxk1Δ mutant (SN791), and an h-d mutant (SN794). Bar, 5 μm.