FIGURE 1.
MGP in rat aorta. A, immunoblot with anti-Gla antibody of extract from calcified aorta (right lane) and GlaMGP purified from bone (left lane). B, immunoblot with anti-Gla antibody of aortas cultured for 3 days in DMEM containing 10 μg/ml of vitamin K1, 25 μm warfarin, or 500 μg/ml of vitamin K1 + 25 μm warfarin. C, immunoblot with anti-GluMGP antibody of aortic extract. D, immunoblot with anti-GluMGP antibody of aortas cultured for 3 days in DMEM without or with 25 μm warfarin. Numbers on immunoblots indicate molecular mass standards in kilodaltons.