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. 2011 Jun 29;286(33):28802–28810. doi: 10.1074/jbc.M111.258160

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Nrk is required for placental development. A, control (XX^Nrk) and Nrk-null (X^NrkX^Nrk) fetuses and placentas at 18.5 dpc. B and C, the weight of control (XY, XX, or XX^Nrk) and Nrk-null (X^NrkY or X^NrkX^Nrk) fetuses (B) and placentas (C) at 12.5–18.5 dpc. The mean ± S.D. are shown (n = 5 for 12.5 dpc, n > 15 for 14.5–18.5 dpc). p < 0.05 (*) and 0.005 (**). D, H&E staining of control (XX^Nrk) and Nrk-null (X^NrkX^Nrk) placentas at 18.5 dpc at low (top) and high (bottom) magnification. D, decidual layer; S, spongiotrophoblast layer; L, labyrinth layer; G, glycogen cells in the spongiotrophoblast layer. The three layers are divided by broken and solid lines. Bars, 1 mm (top) and 200 μm (bottom).