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. 2011 Jun 29;286(33):28802–28810. doi: 10.1074/jbc.M111.258160

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Nrk terminates the proliferation of spongiotrophoblasts. A, in situ hybridization analysis of trophoblast-specific protein-α mRNA in control (XX^Nrk) and Nrk-null (X^NrkX^Nrk) placentas at 18.5 dpc. B, immunofluorescence staining of Ki67 protein in control (XX^Nrk) and Nrk-null (X^NrkX^Nrk) placentas at 14.5 dpc. Cryosections were stained with anti-Ki67 antibody (green) and phalloidin (magenta). Merged images are also shown (right). D, decidual layer; S, spongiotrophoblast layer; L, labyrinth layer. Bars, 1 mm (A) and 100 μm (B).