FIGURE 1.

Endogenously produced LTs are required for optimal phagocytosis of C. albicans. A, LTB₄ and cysLT levels were measured in the supernatant from rat AMs infected with 10:1 live or heat-killed C. albicans after 5, 15, and 30 min as described under “Experimental Procedures.” Data are the mean ± S.E. from three separate experiments. *, p < 0.05 compared with unstimulated AMs; #, p < 0.05 compared with live C. albicans. B, AMs were pretreated with or without the 5-LO inhibitor AA 861 (10 μm) or the FLAP inhibitor MK886 (10 μm) for 10 min and challenged with live C. albicans for 90 min, and the phagocytosis was determined microscopically as described. C, AMs from 5-LO^−/− and WT mice were challenged with heat-killed ^FITCC. albicans for 1 h, and phagocytosis was measured by fluorometry. D, peritoneal macrophages (PM) from 5-LO^−/− and WT mice were challenged with heat-killed ^FITCC. albicans for 1 h, and phagocytosis was measured by fluorometry. E, AMs were pretreated or not for 10 min with the BLT1 antagonist CP 105,696 (10 μm) or the cysLT1 antagonist MK 571 (10 μm) before the addition of live C. albicans for 90 min, and phagocytosis was measured microscopically. in C and D, fluorometric assay was performed, and phagocytic indices were calculated and expressed as a percentage of the control. Data are the mean ± S.E. from 3–5 separate experiments. *, p < 0.05 comparing treated to untreated groups or 5-LO^−/− to WT AMs.