FIGURE 2.

Exogenous LTB₄ and LTD₄ both enhance C. albicans-mediated phagocytosis. Rat AMs were pretreated for 10 min without or with LTB₄ (A) or LTD₄ (B) in the indicated doses before the addition of live C. albicans for 90 min, and phagocytosis was measured microscopically. C, AMs from WT and 5-LO^−/− mice were pretreated or not with 10 nm of LTB₄ and/or LTD₄ for 10 min before the addition of heat-killed ^FITCC. albicans for 90 min, and phagocytosis was determined fluorometrically. D, AMs from WT and 5-LO^−/− mice were pretreated or not with 10 nm of LTB₄ and/or LTD₄ for 10 min before the addition of live C. albicans, and phagocytosis was measured microscopically. Data are the mean ± S.E. from three separate experiments. *, p < 0.05 comparing treated to untreated WT groups or 5-LO^−/− to WT AMs; #, p < 0.05 comparing untreated 5-LO^−/− AMs to LT-activated cells; &, p < 0.05 comparing LTB₄ or LTD₄ alone.