FIGURE 4.

β-arrestin 2 attenuates GPR56ΔNT-stimulated cytotoxicity. Cytotoxicity induced by GPR56 or GPR56ΔNT expression in HEK-293 cells was determined by lactate dehydrogenase secretion in the media at 72 h post-transfection. A significant difference was observed between GPR56 and GPR56ΔNT (**, p < 0.01; n = 6). Cotransfection of HA-β-arrestin 2 resulted in a significant reduction in GPR56ΔNT-dependent cytotoxicity (*, p < 0.03; n = 3), but had no effect on toxicity in cells transfected with the GPR56 wild type. EV, empty vector.