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. 2001 Apr 10;98(8):4758–4763. doi: 10.1073/pnas.071465398

Figure 2.

Figure 2

Characterization of GFP-transfected cells. Cells were transfected with vector 12 h after plating and were analyzed by double immunocytochemistry at 2 DIV. GFP is expressed in both nestin-IR cells (a and a′: arrowhead) and TuJ1-IR cells (b and b′). In another experiment, cells were pulsed for 4 h with 10 μM BrdUrd and processed for GFP/BrdUrd double immunostaining. Cells expressing GFP (FITC labeling) exhibit (c, arrows) or lack (d, arrowheads) BrdUrd labeling (Texas red). (Scale bars = 10 μm.)