Skip to main content
. 2001 Apr 10;98(8):4758–4763. doi: 10.1073/pnas.071465398

Figure 5.

Figure 5

Phorbol ester- and PACAP-induced translocation of PKCγ-GFP fusion protein in cortical cells. (a) PKCγ-GFP fusion protein was visualized throughout the cytoplasm in transfected cortical cells. Note the lack of staining over the eccentric nuclei as indicated by open arrowheads. This pattern was observed in most unstimulated cortical cells transfected with PKCγ-GFP alone, PKCγ-GFP/PAC1short, or PKCγ-GFP/PAC1hop. (b) Activation of PKCγ by 0.2 μM PMA for 15 min induced the translocation of PKCγ-GFP from cytosol to the nucleus (indicated by solid arrowheads). (c) PKCγ-GFP was also visualized throughout the cytoplasm in PKCγ-GFP/PAC1short-transfected cortical cells 45 s after 10 nM PACAP treatment. Open arrowhead indicates absence of prominent nuclear staining. (d) In contrast, PKCγ translocation to the membrane (arrows) and nucleus (solid arrowheads) occurred in PKCγ-GFP/PAC1hop-transfected cells 45 s after 10 nM PACAP. (e) Quantification of PKCγ-GFP nuclear/membrane localization in cells transfected with PKCγ-GFP alone, PKCγ-GFP/PAC1short, or PKCγ-GFP/PAC1hop after 45 s PACAP treatment. Data were obtained from three experiments; *, P < 0.001 vs. GFP-PKC-transfected cells. (Scale bar, 10 μm.)