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. 2001 Apr 10;98(8):4758–4763. doi: 10.1073/pnas.071465398

Figure 6.

Figure 6

PAC1 isoforms differentially regulate DNA synthesis and proliferation. At 2 DIV, transfected cortical precursors were incubated for 6 h with 10 nM PACAP. Cultures were pulsed with BrdUrd (10 μM) for the final 2 h and processed for double GFP/BrdUrd labeling. (a) BrdUrd labeling index of transfected cells. PACAP treatment decreased DNA synthesis in GFP- or GFP/PAC1short-transfected cells, whereas the peptide increased mitosis in GFP/PAC1hop-transfected cells. Values are expressed as % ± SEM (n = 9). Differs from vehicle-treated group: *, P < 0.05; **, P < 0.01. (b) Transfection rates in corresponding cultures. PACAP treatment resulted in an overall increase in GFP-IR cell number (P < 0.05). Values are expressed as % of GFP-IR cells/total ± SEM. (c and d) GFP/PAC1short- or GFP/PAC1hop-transfected cells were treated daily with PACAP for 5 days and processed for GFP immunostaining. (c) Example of a clone at 5 DIV. (Scale bar = 10 μm.) (d) Quantification of GFP-IR clones (≥4 cells) at 5 DIV. Data are expressed as mean number of clones per ten fields ± SEM (n = 8, four experiments). *, unpaired t test, P < 0.02.