TABLE 1.
Plasmids used in this study
aa, amino acids.
| Plasmid | Description | Source |
|---|---|---|
| pPHY1115 | Atg1, endogenous promoter, C-terminal 3× Myc tag, pRS316 | (52) |
| pPHY2728 | Atg1, endogenous promoter, C-terminal 3× Myc tag, pRS413 | This study |
| pPHY3395 | Atg1, endogenous promoter, C-terminal 3× Myc tag, pRS426 | (27) |
| pPHY2869 | Atg1, endogenous promoter, N-terminal 3× HA tag, pRS416 | This study |
| pPHY2376 | Atg1, endogenous promoter, N-terminal 3× HA tag, pRS423 | (53) |
| pPHY2920 | Atg1(K54A), endogenous promoter, N-terminal 3× HA tag, pRS423 | (27) |
| pPHY2925 | Atg1, aa 1–330, endogenous promoter, N-terminal 3× HA tag, pRS423 | This study |
| pPHY3368 | Atg1, aa 331–897, copper-inducible promoter, N-terminal 3× HA tag, pRS426 | This study |
| pPHY2462 | Atg1, aa 1–529, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY2466 | Atg1, aa 1–330, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY2935 | Atg1, aa 331–897, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY2864 | Atg1, aa 448–897, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY2962 | Atg1, aa 550–897, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY2965 | Atg1, aa 600–897, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY2968 | Atg1, aa 650–897, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY2887 | Atg1, aa 731–897, endogenous promoter, C-terminal 3× Myc tag, pRS424 | This study |
| pPHY3381 | Atg1-ZIP, endogenous promoter, N-terminal 3× HA tag, pRS423 | This study |
| pPHY3531 | Atg13, GAL1/10 promoter, N-terminal 6× His tag, pRS426 | This study |
| pPHY2426 | Atg13, CUP1 promoter, N-terminal 3× HA tag, pRS426 | (39) |
| pPHY2806 | Atg17, endogenous promoter, N-terminal 6× Myc tag, pRS424 | (39) |