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Influence of hRFC on hPol δ and hPol ϵ
Reaction mixtures were as described in the legend to Fig. 3A with 50 fmol of hPCNA and 50 fmol of the four-subunit Pol ϵ and 80 fmol of Pol δ where indicated.
| RFC added | hPol ϵ | hPol δ |
|---|---|---|
| nm | pmol nt incorporated/20 min | pmol nt incorporated/20 min |
| 5.6 | 23.7 | 26.7 |
| 0.56 | 13.4 | 11.2 |
| 0.11 | 3.2 | 3.36 |
| None | 0.47 | 0.58 |
