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. 2011 Jun 29;286(33):29005–29013. doi: 10.1074/jbc.M111.230854

FIGURE 1.

FIGURE 1.

Recurrent correlation between HIPK2 and p27kip1 protein expression. A, immunoblot analysis of HIPK2 and p27kip1 in the indicated transiently or stably HIPK2-depleted cells. HIPK2 control cells were obtained by transducing universal negative control siRNA (ctr) or interfering control vector (−CV). γ-Tubulin expression shows equal loading of samples. One of three independent experiments is shown. B, RNAs from H1299-CV and H1299i cells were analyzed by quantitative RT (qRT)-PCR for CDKN1B expression. Each bar represents the mean ± S.D. (lines) of three independent experiments performed in triplicate. C, H1299-CV and H1299i cells were treated with chx and collected at the indicated times. The levels of HIPK2, p27kip1, and p27kip1 phosphorylated at serine 10 were analyzed by WB. γ-Tubulin expression shows equal loading of samples. One representative of three independent experiments is shown. In the lower panel, the graph shows the percentage of remaining p27kip1 protein quantified by scanning densitometry after normalization to the corresponding amount of γ-tubulin. Means ± S.D. (lines) of three independent experiments are shown. *, p < 0.01. 30′, 30 min.