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HIPK2 increases p27kip1 stability by phosphorylating it at serine 10. Shown is the half-life of exogenous wild-type p27-HA, non-phosphorylatable p27S10A-HA, and phosphomimetic p27S10D-HA mutants after protein translation arrest induced by chx in H1299-CV and H1299i cells. The cells were collected at the indicated time, and p27kip1 protein levels were analyzed by WB using anti-HA Abs. γ-Tubulin expression shows equal loading of samples. One of three independent experiments is shown.
