FIGURE 5.
HIPK2 depletion reduces cell proliferation independently from p27kip1. A, TCEs from H1299-CV and H1299i cells transfected with scrambled or p27kip1 antisense oligonucleotides (AS p27) were analyzed by WB for the indicated proteins. γ-Tubulin expression shows equal loading of samples. One representative of three independent experiments is shown. B, proliferation curves comparing H1299-CV and H1299i cells transfected with scrambled or with p27kip1 antisense oligonucleotides (ASp27) counted daily for 6 days. Data are means ± S.D. (lines) of three independent experiments. * denotes a significant difference between H1299 and H1299i cells, and ** denotes a significant difference between H1299 and H1299i cells incubated with p27kip1 antisense oligonucleotides (p < 0.05).