Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 24;286(33):29108–29117. doi: 10.1074/jbc.M111.244905

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Phenotypic analyses of Spink2 mutant mice. A, macroscopic appearance of adult testes from an 8-week-old Spink2^gt^/^gt mouse and a WT littermate (Spink2^+/+). Scale bar, 2 mm. B, comparison of testis weight in Spink2^+/+ and Spink2^gt^/^gt mice. The organs were trimmed of fat and weighed. Values are means ± S.E. (error bars). Statistical significance (*, p < 0.05; n = 8) was assessed using Student's t test. The average testis weight:body weight ratios for WT and Spink2^gt^/^gt males were 0.0071 ± 0.0004 and 0.0055 ± 0.0005, respectively. C, macroscopic appearance of adult epididymis from Spink2^gt^/^gt and Spink2^+/+ mice. Scale bar, 4 mm. D, epididymis weight in Spink2^gt^/^gt mouse and a WT littermate (n = 5). Values are means ± S.E. The average epididymis weight:body weight ratios for WT and Spink2^gt^/^gt males were 0.0025 ± 0.0001 and 0.0025 ± 0.0001, respectively. E, number of mature sperm from Spink2^+/+ and Spink2^gt^/^gt mice. Sperm were collected from the cauda epididymis and vas deferens. Values are means ± S.E. Statistical significance (**, p < 0.01; n = 8) was assessed using Student's t test. The average numbers of mature sperm in WT and Spink2^gt^/^gt males were 2.89 ± 0.40 × 10⁷ and 1.29 ± 0.27 × 10⁷, respectively. F, percentage of sperm from Spink2^+/+ and Spink2^gt^/^gt mice with morphological abnormalities. Sperm were collected from the cauda epididymis and vas deferens. Values are means ± S.E. Statistical significance (*, p < 0.05; n = 6) was assessed using Student's t test. The percentage of abnormal sperm in WT and Spink2^gt^/^gt males was 3.24 ± 0.86% and 17.89 ± 5.78%, respectively. G, morphological abnormalities of Spink2^gt^/^gt sperm. Sperm were collected from the cauda epididymis and vas deferens and observed under a light microscope. The principal morphological abnormality observed was bending at the principal and middle piece junction. Scale bar, 10 μm.