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Automethylation of PRMT1 mutants. Reactions were carried out at 37 °C for 4 h with 15 μm PRMT1 (WT or mutants), 8.57 μm AdoMet (0.9 μm [3H]AdoMet added) in 50 mm sodium phosphate buffer, pH 7.5. The top panel is an SDS-polyacrylamide gel stained with Coomassie Blue to detect total protein, and the bottom panel is an autoradiograph to detect radiolabel incorporation. The gel was exposed to film for 5 days.
