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Competitive methylation experiments of the KRK-CH3 peptide. The reactions were carried out with 100 nm M48L, 60 μm RKK-CH3 peptide as a substrate, 1 μm AdoMet (1 μm [3H]AdoMet added) in 100 mm HEPES buffer at 37 °C, pH 8.0. 20 μm KRK-CH3 peptide were tested as an inhibitor. The filled circles indicate the control group without the KRK-CH3 peptide, and the open circles indicate the test group with KRK-CH3 added.
