TABLE 4.

Peptide substrate specificity of WT-PRMT1 and M48L under steady-state conditions

Peptide substrate	Sequence	Activity of WT-PRMT1	Activity of M48L	Activitya of WT-PRMT1
		s−1	s−1	%
R3	acGGRGGFGGRGGFGGRGG	0.045	0.029	65.9
RKK	acGGRGGFGGKGGFGGKW	0.0051	0.0026	50.5
RKK-CH3	acGGRmeGGFGGKGGFGGKW	0.011	0.0053	47.9
KRK	acKGGFGGRGGFGGKW	0.0071	0.000075	1.06
KRK-CH3	acKGGFGGRmeGGFGGKW	0.0039	0.000023	0.577

^a The methylation velocity is tested under one saturating condition for both AdoMet and peptide substrates, representing the activity of enzymes. The percentage activity is calculated based on the methylation velocity.