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Pre-steady-state kinetic parameters of M48L with WT-PRMT1
| Enzyme | Peptide | Maximal product concentration | kchem |
|---|---|---|---|
| μm | s−1 | ||
| 20 μm WT-PRMT1 | KRK | 20.31 ± 0.25 | 0.020 ± 0.0008 |
| KRK-CH3 | 14.96 ± 1.15 | 0.029 ± 0.0082 | |
| RKK | 14.53 ± 1.82 | 0.073 ± 0.037 | |
| RKK-CH3 | 13.08 ± 0.85 | 0.054 ± 0.016 | |
| 20 μm M48L | KRK | 3.85 ± 0.11 | 0.0087 ± 0.0006 |
| KRK-CH3 | 0.16 ± 0.084 | NDa | |
| RKK | 4.71 ± 0.20 | 0.044 ± 0.008 | |
| RKK-CH3 | 9.37 ± 1.43 | 0.018 ± 0.008 |
a ND stands for not detectable.
