FIGURE 7.

Expression of cell surface glycans and induction of unfolded protein response-mediated ER stress in breast tumor microvasculature. A, WGA staining for N-glycans in microvessels from control and tunicamycin (0–1.0 mg/kg)-treated breast tumor tissue sections. Images were captured under a fluorescence microscope. B, WGA staining of tumor cells. Detection of upr-mediated ER stress in tumor microvasculature. Tumors were fixed, sectioned and stained dually with anti-CD144 (endothelial cell marker) antibody (1:50; v/v) followed by AlexaFluor 488-conjugated secondary antibody (1:100; v/v), and anti-GRP-78 antibody (1:40; v/v) followed by Rhodamin-conjugated secondary antibody (1:100; v/v). C, microvessel (arrows) from peripheral region of breast tissue section immunostained with anti-CD144 antibody (green), and D, microvessel immunostained with anti-GRP-78 antibody (red) were monitored under a fluorescence microscope. E, GRP-78 (red) staining of tumor tissue. Histology scale: 20 μm.