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. 2011 Jun 24;286(33):29139–29145. doi: 10.1074/jbc.M111.267666

FIGURE 4.

FIGURE 4.

Overexpressed TXN stabilizes Txnip protein in 3T3-L1 preadipocytes and inhibits adipogenesis. A, lysates from 3T3-L1s stably overexpressing TXN or GFP introduced by lentivirus (lenti) and then immunostained for Txnip protein after DMI induction. Anti-human TXN immunostaining detected the TXN transgene. B, Txnip protein levels over time after DMI induction in 3T3-L1s stably overexpressing TXN or C35S TXN. C, lysates from 3T3-L1-expressing Txnip and C247S resolved by non-reducing SDS-PAGE and stained with Txnip or TXN antibodies. White lines indicate post-image exchange of lanes at 1 and 12 h to correct loading order. D, adipocyte differentiation in 3T3 stably overexpressing TXN, C32S, or C35S TXN. Lipids were stained with Oil Red O at day 10 after induction.