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Txnip inhibits adipocyte differentiation independent of TXN. A, FLAG epitope-tagged Txnip and C247S double AAXA mutants were stably expressed in 3T3-L1 by lentiviral transduction. Transgene protein levels were assayed by anti-FLAG staining following DMI induction for the indicated time points. B, adipocyte formation for 3T3-L1s stably expressing Txnip mutant transgenes. Lipids were stained with Oil Red O at day 10 after DMI induction.
