FIGURE 5.
Effect of MTS reagents at P2X1 receptor mutants E52C to G96C. A, effect of MTSES (i) and MTSET (ii) on an EC50 concentration of ATP at the P2X1 receptor mutants E52C to G96C. The black lines represent the control response, and the gray lines represent the response in the presence of the MTS reagents (1 mm). B, the response of mutants E52C to G96C to an EC50 concentration of ATP in the presence of MTSES (i) and MTSET (ii). *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, S.E. Conserved residues are highlighted in black.