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Binding of CRM1 basic patch variants to immobilized GST-PKI NES. A, pulldowns were carried out using cell lysates incubated with resin that had been preloaded with GST-PKI NES. B, none of the variants bound to GST alone. C, lysate samples incubated with Ni-NTA show that all of the lysates contained comparable amounts of CRM1. Variant H11 was R553A/R556A, whereas variant H12 was K594A/R596A/R597A.
