FIGURE 7.
SAXS analysis of CRM1 and variants. A, Kratky plot of experimental mouse CRM1 SAXS data overlaid with simulated plots derived from crystal structures of human CRM1 (9), mouse CRM1 (10), and S. cerevisiae Xpo1p (11). B, simulated plots as in A overlaid with experimental S. cerevisiae Xpo1p SAXS data. C, effect on the Kratky plot of human CRM1 (9) of removing the density of the extended C-terminal helix from the crystal structure. D, χ2 values for the comparisons shown in the Kratky plots (A–C). The best fit was obtained between the mouse CRM1 experimental SAXS data and the structure of human CRM1 described by Dong et al. (9). Removing the C-terminal helix from this structure, in which it lies across the toroid, increases χ2 from 5 to 14.2.