FIGURE 2.

ESCRT mutants alter trafficking and degradation patterns of Wsc1*. A, wild type and Δpep4, Δvps27, and Δpep4Δvps27 mutants expressing Wsc1* were processed for indirect immunofluorescence, as described in the legend to Fig. 1B. Cells were visualized by confocal and DIC microscopy. Scale bar, 5 μm. B, a–c display the middle plane of a z-series as described for Fig. 1B captured from Δpep4Δvps27 cells expressing Wsc1*. d–o display the z-stack series from top to bottom. Scale bars, 1 μm. C, Wsc1* expression in wild type and Δpep4, Δvps27, and Δpep4Δvps27 mutants was analyzed by immunoblotting. Membranes were probed with anti-HA antibody (top), stripped, and reprobed with anti-PGK antibody as the loading control (bottom).