FIGURE 2.

Overproduction of Ybp1 inhibits diamide and cycloheximide resistance. A, wild-type cells were transformed with the indicated plasmids. Transformants were tested as above for the ability to tolerate H₂O₂ or diamide. B, a strain lacking Yap1 (yap1Δ) was transformed with the indicated plasmids. Transformants were placed on rich medium containing H₂O₂ to evaluate their relative resistance to this oxidant. C, isogenic wild-type cells or cells overexpressing Ybp1 (TDH3-YBP1) were transformed with an empty high-copy number vector plasmid (YEp351) or the same plasmid containing the wild-type YAP1 gene (YEp351-YAP1). Transformants were grown to mid-log phase and then placed on rich medium containing a gradient of cycloheximide (Cyh).