FIGURE 4.

Wild-type cells transformed with the indicated plasmids were grown to log phase. Transformants were exposed to H₂O₂ and samples were withdrawn at the indicated times. Protein extracts were prepared and equal aliquots were resolved on SDS-PAGE under nonreducing conditions as described (13). Proteins were transferred to nitrocellulose and analyzed by Western blotting using the anti-Yap1 antibody. The positions of the reduced form of Yap1 (Yap1^red) and the H₂O₂-folded form of Yap1 (Yap1^ox) are indicated on the right side.