FIGURE 5.

Oxidative folding of Yap1 responds to Ybp1 dosage. Increased Ybp1 expression inhibits diamide-induced nuclear translocation of Yap1. Wild-type cells were transformed with a low-copy number plasmid expressing GFP-Yap1 and either a vector plasmid (pRS315) or pRS315 carrying the PGK1-YBP1 fusion gene. Transformants were grown to early log phase and then subjected to diamide stress for the times indicated on the left. Cells were withdrawn and visualized for GFP-Yap1 (GFP), DNA (DAPI), or by Nomarski optics (DIC). Quantitation of the fluorescence pattern seen in 200 representative cells is presented at the bottom of the figure.