FIGURE 9.

Ybp1 function is conserved in C. glabrata. A, C. glabrata strains derived from the wild-type (40F1: WT) were placed on YPD medium containing the indicated oxidants. These strains included an isogenic Cgybp1Δ mutant, a strain containing an integrated CgYBP1-HA allele (CgYBP1-HA), or a strain expressing the Ybp1-HA-tagged protein from the CgPGK1 promoter (CgPGK1-CgYBP1-HA). B, whole cell protein extracts were prepared from the strains described above. Equal aliquots of protein were resolved on SDS-PAGE and analyzed by Western blotting using anti-HA antibody. The top panel presents the Western blotting data with the estimated size of CgYbp1-HA indicated on the right. The bottom panel is the same blot stained with Ponceau S prior to Western analysis. Size standards are shown in the most right lane.