TABLE 1.

Net charge (Z) and lytic activity (±) of phage lysins in the absence of their CBD

* Species of the Firmicute phylum are classified as follows: FBBB, Firmicutes (phylum), Bacilli (class), Bacillales (order), and Bacillaceae (family); FBLS, Firmicutes (phylum), Bacilli (class). Lactobacillales (order), and Streptococcaceae (family); FBBL, Firmicutes (phylum), Bacilli (class), and Bacillales (order), and Listeriaceae (family). % identities are with respect to PlyL (amidases) and PlyBa04 (glycosyl hydrolases), catalytic domains only. PC, phosphatidylcholine; LTA, lipoteichoic acid; PGG, PG glycopolymer (uncharged); WTA, wall teichoic acid; SL, S-layer; TUA, techuronic acid; LPS, lipopolysaccharide; intB, internalin B. B. megaterium and B. subtilis have A1γ (DAP) PG and WTA as their major SCWP but express techuronic acid in minimal phosphate media (as does B. subtilis).

** indicates not amidated on DAP. SCWP in red typeface is negatively charged.