FIGURE 2.
Increased association of full-length and truncated 4E-BP1 (tr4E-BP1) with eIF4E in the liver of Ins2Akita/+ mice. The association of full-length 4E-BP1 (A) and tr4E-BP1 (B) with eIF4E was examined by immunoprecipitating eIF4E from liver supernatants and measuring the amount of 4E-BP1 in the immunoprecipitate (IP) by Western blot analysis. C, total eIF4E was measured in WCL by Western blot analysis. D, 4E-BP1 content was assessed by treating supernatants from liver WCL with λ-phosphatase (PPase) followed by Western blot analysis as described under “Experimental Procedures.” O-GlcNAcylation of full-length 4E-BP1 (E) and tr4E-BP1 (F) bound to eIF4E was also measured by Western blot analysis. Representative blots are shown. Values are means + S.E. (n = 5). *, p < 0.05 versus non-diabetic.