FIGURE 5.
Increased expression of GFAT in the liver of Ins2Akita/+ diabetic mice is mediated by hyperglycemia. A, the expression of GFAT in liver supernatants was assessed by Western blot analysis and normalized to GAPDH content. Representative blots are shown. B, RNA was extracted from liver homogenates following a standard TRIzol protocol. GFAT mRNA expression was normalized to an internal GAPDH control. Values are means + S.E. (n = 10). Statistical significance is denoted by the presence of different letters above the bars on the graphs. Bars with different letters are statistically different, p < 0.05.