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12-HETER mediates 12-(S)-HETE-stimulated cell invasion. A, invasion assays for PC-3M cells transfected with si-GPR31 #2, #4, or #5 (control empty vector) constructs in the presence or absence of various concentrations of 12-(S)-HETE. Note that knockdown of 12-HETER abolishes 12-(S)-HETE-stimulated cell invasion. B, invasiveness is indistinguishable between PC-3M cells transfected with si-GPR31 #2, #4, or #5 constructs, following EGF or 10% FBS stimulation.
