TABLE 1.
EC50 of various agonists
The dose-response-stimulation (log[agonist] vs. response, non-linear regression) software of Prism 5 (GraphPad Software Inc) was employed to analyze the [35S]GTPγS coupling data and calculate the EC50 values. R-squared values of CHO/GPR31 cells treated with 12-(S)-HETE, 5-(S)-HETE, 15-(S)-HETE, and Hela or HEK293 cells treated with 12-(S)-HETE are: 0.9460, 0.9454, 0.8822, 0.9754, and 0.9840. n, numbers of experimental repeats. Each experiment has four determinations.
| Ligands | Cell | EC50 | n |
|---|---|---|---|
| 12-(S)-HETE | CHO/GPR31 | 0.28 ± 1.26 | (n = 6) |
| CHO/pcDNA | n.s.b.a | (n = 2) | |
| 12-(R)-HETE | CHO/GPR31 | n.s.b. | (n = 2) |
| CHO/pcDNA | n.s.b. | (n = 2) | |
| 5-(S)-HETE | CHO/GPR31 | 385.7 ± 62b | (n = 2) |
| CHO/pcDNA | n.s.b. | (n = 2) | |
| 15-(S)-HETE | CHO/GPR31 | 42.1 ± 31b | (n = 2) |
| CHO/pcDNA | n.s.b. | (n = 2) | |
| 12-(S)-HETE | Hela/GPR31 | 0.12 | (n = 1) |
| Hela/pcDNA | n.s.b. | (n = 1) | |
| 12-(S)-HETE | HEK293/GPR31 | 0.11 | (n = 1) |
| HEK293/pcDNA | n.s.b. | (n = 1) |
a n.s.b., no specific binding.
b p < 0.05, vs. 12-(S)-HETE-treated CHO/GPR31 cells, t-test.