FIGURE 1.

MMP-13 induction, expression, and activation in MC3T3-E1 cells. A, quantitative real time-PCR of MMP-13, MMP-2, and MMP-14 in MC3T3-E1 cells grown alone or co-cultured with MDA-1833 on Transwell filters. B, Western blot analysis of MMP-13 expression in MC3T3-E1 cell culture supernatants grown alone or co-cultured with MDA-1833 in direct contact, separated on Transwell filters, or after the addition of MDA-1833 cell-conditioned medium (CM). C, Western blot analysis of MMP-13 in MC3T3-E1 culture supernatants of cells grown in the presence of MDA-1833 cell-conditioned medium (CM) with and without concanavalin (Con) A. Western blot and gelatin zymograms of MMP-2 expression are included as controls. Standards (Std) are purified recombinant MMP-13 or MMP-2.