FIGURE 5.

Induction of MMP-13 expression by SAA3 in MC3T3-E1 cells and validation of SAA3 as a novel MMP-13 substrate. A, Western blot analysis of MMP-13 induction in MC3T3-E1 cells incubated between 1.7 and 170 nm recombinant SAA3. Gelatin zymogram indicating MMP-2 expression is included as a control, and standards (Std) are purified recombinant MMP-13 or MMP-2. B, Western blot analysis using anti-SAA3 and anti-His₆ antibodies of MMP-13-cleaved recombinant SAA3. SAA3 was incubated at 37 °C overnight alone or with active recombinant MMP-13 in the presence and absence of the MMPI marimastat.