TABLE 4.
Construct | Yeast cell lysate GSa activity + Glc-6-P | Glycogen accumulation |
---|---|---|
nmol/min/mg | μg/107 cells | |
Vector alone | 0.68 ± 0.33 | ND |
WT | 19.5 ± 3.3 | 32.3 ± 4.5 |
Site-1 | 3.25 ± 0.63 | 5.58 ± 1.28 |
Site-2 | 1.88 ± 0.42 | 14.7 ± 2.7 |
Site-1/Site-2 | 1.87 ± 0.34 | 0.81 ± 0.22 |
Site-4 | 6.65 ± 1.69 | 22.9 ± 5.5 |
a GS, glycogen synthase; ND, not detectable.