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Gö6976 increases the Km of GC-A. Guanylyl cyclase activity was determined for 9 min in 293T-GC-A cell membranes containing 1 μm ANP, 1 mm ATP, and the indicated concentrations of Gö6976 and GTP where n = 4 from two experiments. The Ki for Gö6976 was 1.0 μm.
