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ATP increases the magnitude of Gö6976-dependent inhibition of GC-B. Guanylyl cyclase activity determined in 293T-GC-B membranes containing 1 μm CNP, 1 mm GTP, 10 μm Gö6976, and the indicated ATP concentrations was divided by activities obtained under identical conditions except that the samples lacked Gö6976, and the quotient was multiplied by 100. The resulting data were plotted as a function of the log of the ATP concentration where n = 6.
