FIGURE 4.

A, a and b, NMIIA or gelsolin immunoprecipitates of bead-associated proteins were immunoblotted for gelsolin and NMIIA, respectively. In separate experiments an irrelevant antibody was used for immunoprecipitation, and immunoprecipitates were immunoblotted with NMIIA and gelsolin. c, gelsolin immunoprecipitates of bead-associated proteins were immunoblotted for NMIIA in response to BSA-coated beads in the presence or absence of Ca²⁺. B, gelsolin null cells transfected with FLAG-tagged G1–G6, G1–G3, and G4–G6 constructs were incubated with collagen beads for 30 min. Bead-associated proteins immunoprecipitated (IP) with FLAG antibody were immunoblotted (WB, Western blot) for NMIIA (a). TCL, total cell lysates (b). These observations were made in four different experiments. C, gelsolin null cells transfected with FLAG-tagged G1–G6 and FLAG-tagged empty construct. Cells were incubated with collagen beads for 30 min, and bead-associated proteins were immunoprecipitated with FLAG antibody and then immunoblotted for NMIIB.