FIGURE 5.

A, fura2/AM-loaded gelsolin (Gsn) WT and gelsolin null cells (a) and NMIIA WT and NMIIA null cells (b) showing Ca²⁺ fluxes in response to collagen-coated bead (CCB) binding measured by ratio fluorimetry. B, a and b, Ca²⁺ fluxes in response to collagen bead binding in gelsolin WT and null cells treated with JA (500 nm) and latrunculin B (LatB, 1 μm), respectively. c, intracellular calcium ion responses to collagen-coated beads in latrunculin B (1 μm)-treated NMIIA^−/− cells. d, calcium responses were also measured in gelsolin WT cells after incubation with BSA-coated beads as controls. Data are means ± S.E. of bound collagen beads per cell (n = 100 cells/group).