FIGURE 10.
Desipramine inhibits hERG forward trafficking. A, shown is a pulse-chase analysis of hERG maturation in 35S-labeled HEK/hERG cells under control conditions or after overnight (o/n) incubation with 30 μm desipramine. Radiolabeled hERG was isolated by immunoprecipitation after the chase periods indicated. Arrows indicate the position of fully fg and cg forms of hERG. B, quantitative analysis of time-dependent changes of fg- and cg-hERG densities measured under control conditions or after overnight incubation with 30 μm desipramine (n = 3). C, shown is a pulse-chase experiment performed with 30 μm desipramine present during the chase (ch.) period but not during synthesis of hERG protein. Note the fast suppression of hERG maturation by desipramine. Data are given as the mean ± S.E.