FIGURE 2.
Desipramine suppresses endogenous rERG/IKr currents in NRVM on short term incubation. A, Western blot shows rERG under control conditions after overnight (17 h) exposure to either 10 or 30 μm desipramine and after a 6-h exposure to 30 μm desipramine. B, rERG currents were recorded under control conditions or after a 6-h exposure to 30 μm desipramine. Currents were elicited in symmetric Cs+ solutions using depolarizing test pulses from −60 to + 60 mV. Holding potential was −80 mV. C, quantitative analysis of maximal rERG tail current densities recorded on return to −80mV under control conditions or after a 6-h exposure to 30 μm desipramine (n = 6) is shown. Data are given as the mean ± S.E. Note that current densities were significantly different at p < 0.05 level.