FIGURE 3.
Desipramine increases internalization of cell surface hERG channels. Stably transfected hERG-HAex cells were prelabeled with anti-HA antibody and incubated for 4 h either under control conditions or in the presence of 30 μm desipramine before fixation, permeabilization, and staining with Alexa Fluor 488-conjugated secondary antibody. Shown are confocal fluorescence images (top row) together with corresponding brightfield images with overlaid fluorescence staining (bottom row). Note that desipramine leads to accumulation of cell surface hERG in large intracellular vesicles. Scale bar, 20 μm.