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Two arginine residues facilitate ganglioside binding at the Sia5 position. Various concentrations of recombinant HCR/F proteins were examined for their ability to bind GD1a: wild type (▴), R1111A (▾), R1256A (♦), and R1111A,R1256A (♢). B50 values represent half-maximal binding to ganglioside. All values represent the arithmetic mean ± S.D. of two independent experiments performed in triplicate.
