FIGURE 3.

The distribution of H3K79 di- and trimethylation is enhanced in the region downstream of the IgH enhancer in plasma cells. A, the 11-kb IgH γ2a gene in the two cell lines representing the B cell (membrane IgH > secretory) and plasma cell (sec IgH ≫ membrane) is identical and in the normal Ig locus context, as diagrammed in this panel. Probes used in the subsequent qPCRs are shown. The IgH γ2a-producing cells were fixed, and chromatin immunoprecipitation was performed as described using antibodies to the individual Lys-79 methylations. B and C, B cells (B) and plasma cells (C). %IP, percentage immunoprecipitated.