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. 2011 Jul 29;286(39):33819–33831. doi: 10.1074/jbc.M111.269779

FIGURE 1.

FIGURE 1.

Expression of AT1G78690 in E. coli leads to the accumulation of a lipid that co-migrates with a triacylated lipid. E. coli BLR(DE3)pLysS/pET15b and BLR(DE3)pLysS/pAt1g78690p were grown and induced with IPTG. Lipids were extracted, and the accumulating lipid was purified using preparative TLC as described under “Experimental Procedures.” All lipids were displayed on HPTLC by development in solvent A and visualized by charring with sulfuric acid. Lane 1, lipid extract from induced BLR(DE3)pLysS/pET15b; lane 2, lipid extract from induced BLR(DE3)pLysS/pAt1g78690p; lane 3, purified accumulating lipid; lane 4, N-acyl-PE standard; lane 5, acyl-PG standard. The migration of the major GPLs, PE and PG, are indicated. The asterisk indicates the lipid that accumulates in lipid extracts prepared from cells induced to overexpress At1g78690p.